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Sangon Biotech 50x tae buffer sangon biotech
50x Tae Buffer Sangon Biotech, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tae+buffer/buffer+tae/pm42269619-202-162-165
Average 86 stars, based on 1 article reviews
50x tae buffer sangon biotech - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Electrophoresis:

Article Title: Aptamer-based nanoparticle drug delivery system designed for targeting GPC3-positive liver cancer
Article Snippet: The gel was prepared with 0.25 g of agarose (Sangon Biotech), 3 g of urea (Sangon Biotech), 0.5 μL of GelRed (Sangon Biotech), and 25 mL of 1× Tris-acetate-ethylenediaminetetraacetic acid (TAE) buffer (Sangon Biotech). .. Electrophoresis was conducted at room temperature for 45 min at 80 volts, using 1× TAE buffer and supplemented with 10 μL of loading buffer (8M urea, 10% (m/v) sucrose, 0.025% (m/v) bromophenol blue, 0.025% (m/v) xylene cyanol FF, 0.05% (m/v) SDS) (Sangon Biotech). .. The gel was subsequently analyzed for accuracy and efficiency under UV light using a gel imaging system (Tanon 1600; Tanon, Shanghai, China).

Article Title: Aptamer-Driven DNA Framework Switches for Clearing Programmed Cell Death-Ligand 1 Positive Extracellular Vesicles via Macrophage Recruitment.
Article Snippet: The immune system employs molecular switches to maintain dynamic homeostasis, yet malignant cells often learn from these natural switches and ultimately evade immune surveillance, leading to immune tolerance and tumor deterioration.. Chemically synthetic switches designed to redirect immune signaling pathways are highly desired for reversing this pathological trajectory but are rarely reported.. Herein, we develop a synthetic DNA framework (DF) switch that reprograms macrophage-mediated immune clearance of Programmed Cell Death-Ligand 1 Positive (PD-L1+) extracellular vesicles (EVs) in vivo.

Extraction:

Article Title: DNA dumbbell-switched CRISPR/Cas13a biosensor for detecting human glycosylases with target-initiated cleavage-ligation-transcription cascade
Article Snippet: Human 8-oxoguanine glycosylase 1 (hOGG1) safeguards genomic stability by repairing oxidized DNA lesions through base excision repair.. Given this pivotal role, the malfunction is a key contributor to carcinogenesis, thereby making hOGG1 a valuable biomarker for prognosis and a promising target for early detection and therapy.. Herein, we demonstrated a fluorometric biosensor for hOGG1 that translated target recognition into CRISPR/Cas13a activation via a programmed biochemical circuit. hOGG1 specifically recognized and excised 8oxoguanine (8-oxoG) from 8-oxoG:C base pairs of a dumbbell DNA probe, thereby initiating the mechanism.

Staining:

Article Title: DNA dumbbell-switched CRISPR/Cas13a biosensor for detecting human glycosylases with target-initiated cleavage-ligation-transcription cascade
Article Snippet: Human 8-oxoguanine glycosylase 1 (hOGG1) safeguards genomic stability by repairing oxidized DNA lesions through base excision repair.. Given this pivotal role, the malfunction is a key contributor to carcinogenesis, thereby making hOGG1 a valuable biomarker for prognosis and a promising target for early detection and therapy.. Herein, we demonstrated a fluorometric biosensor for hOGG1 that translated target recognition into CRISPR/Cas13a activation via a programmed biochemical circuit. hOGG1 specifically recognized and excised 8oxoguanine (8-oxoG) from 8-oxoG:C base pairs of a dumbbell DNA probe, thereby initiating the mechanism.

Marker:

Article Title: DNA dumbbell-switched CRISPR/Cas13a biosensor for detecting human glycosylases with target-initiated cleavage-ligation-transcription cascade
Article Snippet: Human 8-oxoguanine glycosylase 1 (hOGG1) safeguards genomic stability by repairing oxidized DNA lesions through base excision repair.. Given this pivotal role, the malfunction is a key contributor to carcinogenesis, thereby making hOGG1 a valuable biomarker for prognosis and a promising target for early detection and therapy.. Herein, we demonstrated a fluorometric biosensor for hOGG1 that translated target recognition into CRISPR/Cas13a activation via a programmed biochemical circuit. hOGG1 specifically recognized and excised 8oxoguanine (8-oxoG) from 8-oxoG:C base pairs of a dumbbell DNA probe, thereby initiating the mechanism.

Article Title: Analysis of microbial diversity of traditional Chinese starters and screening of special flavor yeast for Chinese baked flatbread
Article Snippet: .. The 50 × TAE Buffer, agarose, DNA Marker, 2 × Power Taq PCR Master Mix, and Taq PCR Master Mix were obtained from Sangon Biotech (Shanghai) Co., Ltd. .. The C 7 - C 30 n -alkane mixture standard was acquired from Sigma-Aldrich (Shanghai) Trading Co., Ltd.

Nucleic Acid Electrophoresis:

Article Title: Rapid Authentication of Flowers of Panax ginseng and Panax notoginseng Using High-Resolution Melting (HRM) Analysis
Article Snippet: .. The products were resolved by 1.5% ( w / v ) agarose (Sangon Biotech Co., Ltd., Shanghai, China) gel electrophoresis in 1× TAE buffer (Sangon Biotech Co., Ltd., Shanghai, China) to confirm target specificity and fragment purity. ..

Agarose Gel Electrophoresis:

Article Title: Aptamer-Driven DNA Framework Switches for Clearing Programmed Cell Death-Ligand 1 Positive Extracellular Vesicles via Macrophage Recruitment.
Article Snippet: The immune system employs molecular switches to maintain dynamic homeostasis, yet malignant cells often learn from these natural switches and ultimately evade immune surveillance, leading to immune tolerance and tumor deterioration.. Chemically synthetic switches designed to redirect immune signaling pathways are highly desired for reversing this pathological trajectory but are rarely reported.. Herein, we develop a synthetic DNA framework (DF) switch that reprograms macrophage-mediated immune clearance of Programmed Cell Death-Ligand 1 Positive (PD-L1+) extracellular vesicles (EVs) in vivo.

Polymerase Chain Reaction:

Article Title: Analysis of microbial diversity of traditional Chinese starters and screening of special flavor yeast for Chinese baked flatbread
Article Snippet: .. The 50 × TAE Buffer, agarose, DNA Marker, 2 × Power Taq PCR Master Mix, and Taq PCR Master Mix were obtained from Sangon Biotech (Shanghai) Co., Ltd. .. The C 7 - C 30 n -alkane mixture standard was acquired from Sigma-Aldrich (Shanghai) Trading Co., Ltd.

Sequencing:

Article Title: Assessment of the thermal stability and iron saturation level of lactoferrin via intrinsic fluorescence measurements of melting temperature.
Article Snippet: Agarose (molecular biology grade) and SYPRO orange dye (SO, 5000 × ) were obtained from Invitrogen (Carlsbad, CA). .. All DNA sequence (Table S1) and the TAE buffer (50 × ; 2 mol/L Tris-Acetate; 50 mmol/L EDTA; pH 8.0–8.6) were purchased from Sangon Bio-Tech Co. Ltd. (Shanghai, China). .. Heparin sodium was obtained from Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

other:

Article Title: Accurate detection of Cd (II) based on His-Pd@Apt/PANI@Au-modified needle electrochemical ratiometric/colorimetric dual-mode sensor combined with a WeChat mini program.
Article Snippet: Heavy metal cadmium (Cd (II)) contamination in aquatic products poses severe health risks, necessitating rapid, accurate, and portable detection methods.. Traditional electrochemical sensors often suffer from background interference and low accuracy, especially when utilizing stainless steel acupuncture needles as electrodes.. Singlesignal systems are prone to environmental fluctuations, limiting reliability.



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